FadA

• Description: acetyl-CoA C-acyltransferase
  
  

Gene name	fadA
Synonyms	yusK
Essential	no
Product	acetyl-CoA C-acyltransferase
Function	fatty acid degradation
Gene expression levels in SubtiExpress: fadA
Metabolic function and regulation of this protein in SubtiPathways: fadA
MW, pI	40 kDa, 5.184
Gene length, protein length	1173 bp, 391 aa
Immediate neighbours	fadE, fadN
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of lipids, sporulation/ other

This gene is a member of the following regulons

CcpA regulon, FadR regulon, SdpR regulon

The gene

Basic information

• Locus tag: BSU32830

Database entries

• BsubCyc: BSU32830

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA (according to Swiss-Prot)

• Protein family: thiolase family (according to Swiss-Prot)

• Paralogous protein(s): MmgA, YhfS

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU32830

• Structure: 3SS6 (the protein of B. anthracis, 41% identity, 73% similarity)

• UniProt: O32177

• KEGG entry: [3]

• E.C. number: 2.3.1.16

Expression and regulation

• Operon:
  • fadM-fadN-fadA-fadE PubMed
  • fadN-fadA-fadE PubMed

• Expression browser: fadA PubMed

• Sigma factor:
  • fadN: SigA PubMed

• Regulation:
  • fadN: induced by long-chain fatty acids (FadR) PubMed
  • fadN: subject to carbon catabolite repression (CcpA-HPr(Ser-P) PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed
  • SdpR: transcription activation PubMed
  • CcpA-HPr(Ser-P): transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 37 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)

José E González-Pastor, Errett C Hobbs, Richard Losick
Cannibalism by sporulating bacteria.
Science: 2003, 301(5632);510-3
[PubMed:12817086] [WorldCat.org] [DOI] (I p)