WapA

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  • Description: cell wall-associated protein precursor, contact-dependent growth inhibition protein

Gene name wapA
Synonyms
Essential no
Product cell wall-associated protein precursor
Function intercellular competition
Gene expression levels in SubtiExpress: wapA
Interactions involving this protein in SubtInteract: WapA
MW, pI 258 kDa, 6.131
Gene length, protein length 7002 bp, 2334 aa
Immediate neighbours yxxG, yxxF
Sequences Protein DNA DNA_with_flanks
Genetic context
WapA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
WapA expression.png















Categories containing this gene/protein

cell wall/ other, toxins, antitoxins and immunity against toxins

This gene is a member of the following regulons

DegU regulon, WalR regulon, YvrHb regulon

The gene

Basic information

  • Locus tag: BSU39230

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relexed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • the C-terminal toxic domain has RNase activity (cleaves tRNAs) PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • cell wall binding domain as retention signal
    • C-terminal toxic domain with RNase activity (cleaves tRNAs) PubMed
  • Modification:
  • Effectors of protein activity:
  • Localization:
    • extracellular (signal peptide), cell wall binding domain as retention signal, major constituent of the secretome PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed at high salt concentration (DegU-P) PubMed
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 66 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 6 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 51 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References