yxxF

168

unknown

locus: BSU_39240

Molecular weight: 34.16 kDa

pI: 7.96

Protein length: 311 aa

Gene length: 936 bp

function: unknown

product: unknown

essential: no

ec: null

synonyms: yxxF

List of homologs in different organisms, belongs to COG0697 (Galperin et al., 2021)

Gene

Coordinates: 4,030,710 → 4,031,645

The protein

Protein family

• [wiki|EamA transporter family] (according to UniProt)

[wiki|Domains]

• 2 [wiki|EamA domain]s (aa 24-147, aa 166-292) (according to UniProt)

Structure

• [AF|Q07835]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|AFED0CECAD2F9948049612CE8BC890938A0857FB|yxxF]

  description: [Pubmed|10746760]

Biological materials

Mutant

• MGNA-B718 (yxxF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1717 NBRP B. subtilis, Japan]
• BKE39240 (Δ[gene|AFED0CECAD2F9948049612CE8BC890938A0857FB|yxxF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39240 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAAACGTATTCTACCTC,  downstream forward: _UP4_TAAAAAATGTAAAAAGGCCT
• BKK39240 (Δ[gene|AFED0CECAD2F9948049612CE8BC890938A0857FB|yxxF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39240 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAAACGTATTCTACCTC,  downstream forward: _UP4_TAAAAAATGTAAAAAGGCCT

Expression system

• GP4623 (trpC2 xkdE::P_xyl-[gene|AFED0CECAD2F9948049612CE8BC890938A0857FB|yxxF]-ermC), available in [wiki|Jörg Stülke]'s lab

References

• 10746760