RibA

• Description: GTP cyclohydrolase II/ 3,4-dihydroxy-2-butanone 4-phosphate synthase
  
  

Gene name	ribA
Synonyms
Essential	no
Product	GTP cyclohydrolase II/ 3,4-dihydroxy-2-butanone 4-phosphate synthase
Function	riboflavin biosynthesis
Gene expression levels in SubtiExpress: ribA
Metabolic function and regulation of this protein in SubtiPathways: ribA
MW, pI	43 kDa, 5.582
Gene length, protein length	1194 bp, 398 aa
Immediate neighbours	ribH, ribE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

FMN-box

The gene

Basic information

• Locus tag: BSU23260

Database entries

• BsubCyc: BSU23260

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU23260

• Structure: 2BZ0 (from E. coli, 54% identity, 69% similarity to the C-terminal domain) PubMed

• UniProt: P17620

• KEGG entry: [3]

• E.C. number: 3.5.4.25, 4.1.99.12

Expression and regulation

• Operon: ribD-ribE-ribA-ribH-ribT PubMed

• Expression browser: ribA PubMed

• Sigma factor: SigA PubMed

• Regulation: expressed in the absence of FMN (FMN-box) PubMed

• Regulatory mechanism: FMN-box: riboswitch, mediates termination/ antitermination control of the operon, in the absence of FMN: antitermination, in the presence of FMN: termination PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 199 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 947 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: