ThdF

• Description: GTP-binding protein, putative tRNA modification GTPase
  
  

Gene name	thdF
Synonyms	mnmE
Essential	no
Product	putative tRNA modification GTPase
Function	tRNA modification
Gene expression levels in SubtiExpress: thdF
Interactions involving this protein in SubtInteract: ThdF
MW, pI	50 kDa, 4.643
Gene length, protein length	1377 bp, 459 aa
Immediate neighbours	gidA, jag
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, GTP-binding proteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU41020

Database entries

• BsubCyc: BSU41020

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP

• Protein family: MnmE subfamily (according to Swiss-Prot) Era/Obg family

• Paralogous protein(s): Era, Obg, YphC, YsxC, RbgA, YqeH, ThdF, YyaF, YnbA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GidA-ThdF (suggested by interaction of E. coli counterparts PubMed and by co-localization on the chromosome)

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU41020

• Structure:

• UniProt: P25811

• KEGG entry: [3]

• E.C. number:

Additional information

Information on the corresponding protein from E. coli: EcoCyc MnmE required for wild-type 5-methylaminomethyl-2-thiouridine modification of tRNA, interacts with GidA. MnmE also appears to play a role in oxidation of thiophene and furan compounds and regulates glutamate-dependent acid resistance.

Expression and regulation

• Operon: thdF-gidA-rsmG-noc PubMed

• Expression browser: thdF PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 180 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 507 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan