MurB

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  • Description: UDP-N-acetylenolpyruvoylglucosamine reductase

Gene name murB
Synonyms ylxC
Essential yes PubMed
Product UDP-N-acetylenolpyruvoylglucosamine reductase
Function peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: murB
Metabolic function and regulation of this protein in SubtiPathways:
murB
MW, pI 32 kDa, 6.087
Gene length, protein length 909 bp, 303 aa
Immediate neighbours murG, divIB
Sequences Protein DNA DNA_with_flanks
Genetic context
MurB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MurB expression.png




























Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, sporulation proteins, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

SigE regulon, SigM regulon, SpoIIID regulon

The gene

Basic information

  • Locus tag: BSU15230

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH (according to Swiss-Prot)
  • Protein family: FAD-binding PCMH-type domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2GQU (from Thermus caldophilus, 38% identity, 55% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell (SigE) PubMed
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1522 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1652 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Adriano Henriques, Lisbon, Portugal homepage

Your additional remarks

References

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

Jean van Heijenoort
Lipid intermediates in the biosynthesis of bacterial peptidoglycan.
Microbiol Mol Biol Rev: 2007, 71(4);620-35
[PubMed:18063720] [WorldCat.org] [DOI] (P p)

Gonçalo Real, Adriano O Henriques
Localization of the Bacillus subtilis murB gene within the dcw cluster is important for growth and sporulation.
J Bacteriol: 2006, 188(5);1721-32
[PubMed:16484183] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

S L Rowland, J Errington, R G Wake
The Bacillus subtilis cell-division 135-137 degrees region contains an essential orf with significant similarity to murB and a dispensable sbp gene.
Gene: 1995, 164(1);113-6
[PubMed:7590298] [WorldCat.org] [DOI] (P p)

G Theeragool, A Miyao, K Yamada, T Sato, Y Kobayashi
In vivo expression of the Bacillus subtilis spoVE gene.
J Bacteriol: 1993, 175(13);4071-80
[PubMed:8320223] [WorldCat.org] [DOI] (P p)

A O Henriques, H de Lencastre, P J Piggot
A Bacillus subtilis morphogene cluster that includes spoVE is homologous to the mra region of Escherichia coli.
Biochimie: 1992, 74(7-8);735-48
[PubMed:1391053] [WorldCat.org] [DOI] (P p)