CysI

• Description: sulfite reductase (NADPH2)
  
  

Gene name	cysI
Synonyms	yvgR
Essential	no
Product	sulfite reductase (NADPH2)
Function	sulfite reduction
Gene expression levels in SubtiExpress: cysI
Metabolic function and regulation of this protein in SubtiPathways: cysI
MW, pI	67 kDa, 4.573
Gene length, protein length	1815 bp, 605 aa
Immediate neighbours	cysJ, helD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sulfur metabolism

This gene is a member of the following regulons

CysL regulon

The gene

Basic information

• Locus tag: BSU33440

Phenotypes of a mutant

• unable to grow with sulfate or sulfite as the only sulfur source PubMed

Database entries

• BsubCyc: BSU33430

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU33430

• Structure:

• UniProt: O32213

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cysI-cysJ PubMed

• Expression browser: cysI PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by sulfite, sulfate, and thiosulfate (CysL) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism:
  • CysL: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1772 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1407 PubMed

Biological materials

• Mutant:
  • 1A802 ( cysI::kan), PubMed, available at BGSC
  • 1A934 ( cysI::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: