CypX

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Gene name cypX
Synonyms
Essential no
Product cytochrome P450, cyclo-l-leucyl-l-leucyl dipeptide oxidase
Function biosynthesis of the extracellular iron chelate pulcherrimin
Gene expression levels in SubtiExpress: cypX
MW, pI 45 kDa, 5.306
Gene length, protein length 1215 bp, 405 aa
Immediate neighbours yvnA, yvmC
Sequences Protein DNA DNA_with_flanks
Genetic context
CypX context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CypX expression.png















Categories containing this gene/protein

acquisition of iron, electron transport/ other, iron metabolism

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

  • Locus tag: BSU35060

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • implicated in the three-step oxidative transformation of the diketopiperazine cyclo-l-leucyl-l-leucyl into pulcherriminic acid PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Max J Cryle, Stephen G Bell, Ilme Schlichting
Structural and biochemical characterization of the cytochrome P450 CypX (CYP134A1) from Bacillus subtilis: a cyclo-L-leucyl-L-leucyl dipeptide oxidase.
Biochemistry: 2010, 49(34);7282-96
[PubMed:20690619] [WorldCat.org] [DOI] (I p)

R L Uffen, E Canale-Parola
Synthesis of pulcherriminic acid by Bacillus subtilis.
J Bacteriol: 1972, 111(1);86-93
[PubMed:4204912] [WorldCat.org] [DOI] (P p)

additional paper