MenC

• Description: O-succinylbenzoate synthase
  
  

Gene name	menC
Synonyms	ytfD
Essential	yes PubMed
Product	O-succinylbenzoate synthase
Function	biosynthesis of menaquinone
Gene expression levels in SubtiExpress: menC
Metabolic function and regulation of this protein in SubtiPathways: menC
MW, pI	41 kDa, 5.888
Gene length, protein length	1113 bp, 371 aa
Immediate neighbours	mntA, menE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, essential genes

The gene

Basic information

• Locus tag: BSU30780

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU30780

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H₂O (according to Swiss-Prot)

• Protein family: mandelate racemase/muconate lactonizing enzyme family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU30780

• Structure:

• UniProt: O34514

• KEGG entry: [3]

• E.C. number: 4.2.1.113

Expression and regulation

• Operon:
  • menF-menD-ytxM-menB-menE-menC PubMed
  • menB-menE-menC PubMed
  • menE-menC PubMed

• Expression browser: menC PubMed

• Sigma factor:
  • menF: SigA PubMed
  • menB: SigA PubMed
  • menE: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • A ncRNA is predicted between mntA and menC PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

D R Palmer, J B Garrett, V Sharma, R Meganathan, P C Babbitt, J A Gerlt
Unexpected divergence of enzyme function and sequence: "N-acylamino acid racemase" is o-succinylbenzoate synthase.
Biochemistry: 1999, 38(14);4252-8
[PubMed:10194342] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)

X Qin, H W Taber
Transcriptional regulation of the Bacillus subtilis menp1 promoter.
J Bacteriol: 1996, 178(3);705-13
[PubMed:8550504] [WorldCat.org] [DOI] (P p)

J R Driscoll, H W Taber
Sequence organization and regulation of the Bacillus subtilis menBE operon.
J Bacteriol: 1992, 174(15);5063-71
[PubMed:1629163] [WorldCat.org] [DOI] (P p)