Phy

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  • Description: phytase

Gene name phy
Synonyms yodV, yzxA
Essential no
Product 3-phytase
Function untilization of inositol hexakisphosphate (phytate)
Gene expression levels in SubtiExpress: phy
MW, pI 41 kDa, 4.936
Gene length, protein length 1146 bp, 382 aa
Immediate neighbours cgeB, yodU
Sequences Protein DNA DNA_with_flanks
Genetic context
Phy context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Phy expression.png















Categories containing this gene/protein

phosphate metabolism

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

  • Locus tag: BSU19800

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate (according to Swiss-Prot)
  • Protein family: msrA Met sulfoxide reductase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • the gene is not expressed in B. subtilis due to the absene of a functional promoter PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Rim Tinhinen Maougal, Adnane Bargaz, Charaf Sahel, Laurie Amenc, Abdelhamid Djekoun, Claude Plassard, Jean-Jacques Drevon
Localization of the Bacillus subtilis beta-propeller phytase transcripts in nodulated roots of Phaseolus vulgaris supplied with phytate.
Planta: 2014, 239(4);901-8
[PubMed:24407511] [WorldCat.org] [DOI] (I p)

Ameny Farhat-Khemakhem, Mamdouh Ben Ali, Ines Boukhris, Bassem Khemakhem, Emmanuelle Maguin, Samir Bejar, Hichem Chouayekh
Crucial role of Pro 257 in the thermostability of Bacillus phytases: biochemical and structural investigation.
Int J Biol Macromol: 2013, 54;9-15
[PubMed:23178368] [WorldCat.org] [DOI] (I p)

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Martha Guerrero-Olazarán, Lilí Rodríguez-Blanco, J Gerardo Carreon-Treviño, Juan A Gallegos-López, José M Viader-Salvadó
Expression of a Bacillus phytase C gene in Pichia pastoris and properties of the recombinant enzyme.
Appl Environ Microbiol: 2010, 76(16);5601-8
[PubMed:20601512] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Oliwia Makarewicz, Sarah Dubrac, Tarek Msadek, Rainer Borriss
Dual role of the PhoP approximately P response regulator: Bacillus amyloliquefaciens FZB45 phytase gene transcription is directed by positive and negative interactions with the phyC promoter.
J Bacteriol: 2006, 188(19);6953-65
[PubMed:16980498] [WorldCat.org] [DOI] (P p)

N C Ha, B C Oh, S Shin, H J Kim, T K Oh, Y O Kim, K Y Choi, B H Oh
Crystal structures of a novel, thermostable phytase in partially and fully calcium-loaded states.
Nat Struct Biol: 2000, 7(2);147-53
[PubMed:10655618] [WorldCat.org] [DOI] (P p)

S Roels, R Losick
Adjacent and divergently oriented operons under the control of the sporulation regulatory protein GerE in Bacillus subtilis.
J Bacteriol: 1995, 177(21);6263-75
[PubMed:7592393] [WorldCat.org] [DOI] (P p)