SpoVD

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Gene name spoVD
Synonyms
Essential no
Product penicillin-binding protein (spore cortex)
Function spore morphogenesis
Gene expression levels in SubtiExpress: spoVD
Interactions involving this protein in SubtInteract: SpoVD
MW, pI 71 kDa, 8.85
Gene length, protein length 1935 bp, 645 aa
Immediate neighbours pbpB, murE
Sequences Protein DNA DNA_with_flanks
Genetic context
SpoVD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpoVD expression.png















Categories containing this gene/protein

cell wall synthesis, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon, SpoIIID regulon

The gene

Basic information

  • Locus tag: BSU15170

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: protects SpoVE from proteolytic degradation PubMed
  • Protein family:
  • Paralogous protein(s): PbpB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: intramolecular disulfide bonds between two Cys residues are reduced by StoA, this is rquired for activity of SpoVD PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed during sporulation in the mother cell (SigE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original Publications

Ewa Bukowska-Faniband, Lars Hederstedt
Cortex synthesis during Bacillus subtilis sporulation depends on the transpeptidase activity of SpoVD.
FEMS Microbiol Lett: 2013, 346(1);65-72
[PubMed:23789716] [WorldCat.org] [DOI] (I p)

Allison Fay, Pablo Meyer, Jonathan Dworkin
Interactions between late-acting proteins required for peptidoglycan synthesis during sporulation.
J Mol Biol: 2010, 399(4);547-61
[PubMed:20417640] [WorldCat.org] [DOI] (I p)

Yiming Liu, Mirja Carlsson Möller, Lise Petersen, Christopher A G Söderberg, Lars Hederstedt
Penicillin-binding protein SpoVD disulphide is a target for StoA in Bacillus subtilis forespores.
Mol Microbiol: 2010, 75(1);46-60
[PubMed:19919673] [WorldCat.org] [DOI] (I p)

Dirk-Jan Scheffers
Dynamic localization of penicillin-binding proteins during spore development in Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 3);999-1012
[PubMed:15758244] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

B Zhang, R A Daniel, J Errington, L Kroos
Bacillus subtilis SpoIIID protein binds to two sites in the spoVD promoter and represses transcription by sigmaE RNA polymerase.
J Bacteriol: 1997, 179(3);972-5
[PubMed:9006059] [WorldCat.org] [DOI] (P p)

R A Daniel, S Drake, C E Buchanan, R Scholle, J Errington
The Bacillus subtilis spoVD gene encodes a mother-cell-specific penicillin-binding protein required for spore morphogenesis.
J Mol Biol: 1994, 235(1);209-20
[PubMed:8289242] [WorldCat.org] [DOI] (P p)

R A Daniel, J Errington
DNA sequence of the murE-murD region of Bacillus subtilis 168.
J Gen Microbiol: 1993, 139(2);361-70
[PubMed:8436954] [WorldCat.org] [DOI] (P p)