NucA

• Description: membrane-associated nuclease, catalyzes DNA cleavage during transformation
  
  

Gene name	nucA
Synonyms	comI
Essential	no
Product	membrane-associated nuclease
Function	genetic transformation, DNA uptake
Gene expression levels in SubtiExpress: nucA
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	16 kDa, 4.677
Gene length, protein length	441 bp, 147 aa
Immediate neighbours	nin, tlpC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, membrane proteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU03430

Database entries

• BsubCyc: BSU03430

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • integral membrane protein PubMed

Database entries

• BsubCyc: BSU03430

• Structure:

• UniProt: P12667

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nucA-nin PubMed

• Expression browser: nucA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism: ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hanne Jarmer, Randy Berka, Steen Knudsen, Hans H Saxild
Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions.
FEMS Microbiol Lett: 2002, 206(2);197-200
[PubMed:11814663] [WorldCat.org] [DOI] (P p)

R Provvedi, I Chen, D Dubnau
NucA is required for DNA cleavage during transformation of Bacillus subtilis.
Mol Microbiol: 2001, 40(3);634-44
[PubMed:11359569] [WorldCat.org] [DOI] (P p)

D van Sinderen, R Kiewiet, G Venema
Differential expression of two closely related deoxyribonuclease genes, nucA and nucB, in Bacillus subtilis.
Mol Microbiol: 1995, 15(2);213-23
[PubMed:7746143] [WorldCat.org] [DOI] (P p)