CwlJ

• Description: spore coat protein, cell wall hydrolase
  
  

Gene name	cwlJ
Synonyms	ycbQ
Essential	no
Product	spore coat protein, cell wall hydrolase
Function	spore germination
Gene expression levels in SubtiExpress: cwlJ
MW, pI	16 kDa, 9.354
Gene length, protein length	426 bp, 142 aa
Immediate neighbours	ycbP, ycbR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall/ other, sporulation proteins, germination

This gene is a member of the following regulons

SigE regulon, SigK regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU02600

Database entries

• BsubCyc: BSU02600

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: cwlJ family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • outer edge of the spore cortex PubMed
  • inner spore coat, localization depends on SafA PubMed

Database entries

• BsubCyc: BSU02600

• Structure:

• UniProt: P42249

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cwlJ PubMed

• Expression browser: cwlJ PubMed

• Sigma factor: SigE PubMed, SigK PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigE, SigK, SpoIIID) PubMed

• Regulatory mechanism:
  • SpoIIID: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1G19 ( cwlJ::tet), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Peter T McKenney, Adam Driks, Patrick Eichenberger
The Bacillus subtilis endospore: assembly and functions of the multilayered coat.
Nat Rev Microbiol: 2013, 11(1);33-44
[PubMed:23202530] [WorldCat.org] [DOI] (I p)

Original publications

B Setlow, J Yu, Y-Q Li, P Setlow
Analysis of the germination kinetics of individual Bacillus subtilis spores treated with hydrogen peroxide or sodium hypochlorite.
Lett Appl Microbiol: 2013, 57(4);259-65
[PubMed:23746146] [WorldCat.org] [DOI] (I p)

Yunfeng Li, Xuan Y Butzin, Andrew Davis, Barbara Setlow, George Korza, Fatma Işik Üstok, Graham Christie, Peter Setlow, Bing Hao
Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination.
J Bacteriol: 2013, 195(11);2530-40
[PubMed:23543708] [WorldCat.org] [DOI] (I p)

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Xuan Yi, Peter Setlow
Studies of the commitment step in the germination of spores of bacillus species.
J Bacteriol: 2010, 192(13);3424-33
[PubMed:20435722] [WorldCat.org] [DOI] (I p)

Alicia Monroe, Peter Setlow
Localization of the transglutaminase cross-linking sites in the Bacillus subtilis spore coat protein GerQ.
J Bacteriol: 2006, 188(21);7609-16
[PubMed:16936016] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

Katerina Ragkousi, Peter Setlow
Transglutaminase-mediated cross-linking of GerQ in the coats of Bacillus subtilis spores.
J Bacteriol: 2004, 186(17);5567-75
[PubMed:15317760] [WorldCat.org] [DOI] (P p)

Haridasan Chirakkal, Michele O'Rourke, Abdelmadjid Atrih, Simon J Foster, Anne Moir
Analysis of spore cortex lytic enzymes and related proteins in Bacillus subtilis endospore germination.
Microbiology (Reading): 2002, 148(Pt 8);2383-2392
[PubMed:12177332] [WorldCat.org] [DOI] (P p)

Irina Bagyan, Peter Setlow
Localization of the cortex lytic enzyme CwlJ in spores of Bacillus subtilis.
J Bacteriol: 2002, 184(4);1219-24
[PubMed:11807087] [WorldCat.org] [DOI] (P p)

M Paidhungat, K Ragkousi, P Setlow
Genetic requirements for induction of germination of spores of Bacillus subtilis by Ca(2+)-dipicolinate.
J Bacteriol: 2001, 183(16);4886-93
[PubMed:11466292] [WorldCat.org] [DOI] (P p)

S Ishikawa, K Yamane, J Sekiguchi
Regulation and characterization of a newly deduced cell wall hydrolase gene (cwlJ) which affects germination of Bacillus subtilis spores.
J Bacteriol: 1998, 180(6);1375-80
[PubMed:9515903] [WorldCat.org] [DOI] (P p)