TenA

• Description: thiaminase II
  
  

Gene name	tenA
Synonyms
Essential	no
Product	thiaminase II
Function	thiamine salvage
Gene expression levels in SubtiExpress: tenA
Metabolic function and regulation of this protein in SubtiPathways: tenA
MW, pI	27 kDa, 4.99
Gene length, protein length	708 bp, 236 aa
Immediate neighbours	yjbQ, tenI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, most abundant proteins

This gene is a member of the following regulons

Thi-box

The gene

Basic information

• Locus tag: BSU11650

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Thiamine + H₂O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole (according to Swiss-Prot)

• Protein family: thiaminase-2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1YAF, 2QCX (complex with formyl aminommethyl pyrimidine)

• UniProt: P25052

• KEGG entry: [3]

• E.C. number: 3.5.99.2

Expression and regulation

• Operon: tenA-tenI-thiO-thiS-thiG-thiF-thiD PubMed

• Expression browser: tenA PubMed

• Sigma factor:

• Regulation:
  • repressed by thiamine (Thi-box) PubMed

• Regulatory mechanism:
  • Thi-box: RNA switch PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

T P Begley, D M Downs, S E Ealick, F W McLafferty, A P Van Loon, S Taylor, N Campobasso, H J Chiu, C Kinsland, J J Reddick, J Xi
Thiamin biosynthesis in prokaryotes.
Arch Microbiol: 1999, 171(5);293-300
[PubMed:10382260] [WorldCat.org] [DOI] (P p)