KinA

• Description: two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay
  
  

Gene name	kinA
Synonyms	spoIIF, spoIIJ, gsiC, scoB, scoD
Essential	no
Product	two-component sensor kinase
Function	initiation of sporulation
Gene expression levels in SubtiExpress: kinA
Interactions involving this protein in SubtInteract: KinA
Function and regulation of this protein in SubtiPathways: kinA
MW, pI	68 kDa, 5.491
Gene length, protein length	1818 bp, 606 aa
Immediate neighbours	pbpH, patA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, phosphorelay, phosphoproteins

This gene is a member of the following regulons

SigH regulon, Spo0A regulon, stringent response

The gene

Basic information

• Locus tag: BSU13990

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • autophosphorylation, phosphorylation of Spo0F
  • mainly active in the older, inner regions of a colony (with KinB) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • three tandem PAS domains in the N-terminal region of KinA, the second PAS domain is the major N-terminal determinant of KinA dimerization PubMed
  • the first PAS domain is required for NAD(+) binding PubMed
  • C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:
  • interaction with KipI or Sda inhibits autophosphorylation of KinA PubMed
  • autophosphorylation is inhibited by SivA and BslA PubMed
  • activated by interaction with NAD(+) that indicated poor respiratory activity of the cell PubMed

• Interactions:
  • forms tetramers PubMed
  • KinA-KipI PubMed
  • KinA-Sda PubMed
  • Spo0F-KinA

• Localization:
  • cytoplasm

Database entries

• Structure: 2VLG (PAS domain)

• UniProt: P16497

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: kinA PubMed

• Expression browser: kinA PubMed

• Sigma factor: SigH PubMed

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • induced upon addition of decoyinine (positive stringent response) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Liza Gross
Built-in timer delays differentiation.
PLoS Biol: 2012, 10(1);e1001254
[PubMed:22303284] [WorldCat.org] [DOI] (I p)