ManP

• Description: trigger enzyme: mannose-specific phosphotransferase system, EIIBCA of the PTS
  
  

Gene name	manP
Synonyms	yjdD
Essential	no
Product	trigger enzyme: mannose-specific phosphotransferase system, EIIBCA
Function	mannose uptake and phosphorylation, control of ManR activity
Gene expression levels in SubtiExpress: manP
Interactions involving this protein in SubtInteract: ManP
Metabolic function and regulation of this protein in SubtiPathways: manP
MW, pI	61 kDa, 6.715
Gene length, protein length	1767 bp, 589 aa
Immediate neighbours	manR, manA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, transcription factors and their control, trigger enzyme, membrane proteins, phosphoproteins

This gene is a member of the following regulons

ManR regulon

The gene

Basic information

• Locus tag: BSU12010

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transport and concomitant phosphorylation of mannose

• Protein family: PTS permease, fructose/ mannitol permease (Fru) family PubMed

• Paralogous protein(s): FruA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-365 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ManP-ManR, ManP-HPr

• Localization: membrane

Database entries

• Structure: 2R48 (IIB domain)

• UniProt: O31645

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: manP-manA-yjdF PubMed

• Expression browser: manP PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by mannose (ManR) PubMed
  • repressed by glucose (ManR) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • ManR: transcription activation in the presence of mannose and absence of glucose PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: