manR

168

transcriptional activator of the manP-manA-yjdF operon

Locus: BSU_12000

Molecular weight: 75.13 kDa

Isoelectric point: 8.04

Protein length: 648 aa

Gene length: 1947 bp

Function: regulation of mannose utilization

Product: transcriptional activator, PRD-type

Essential: no

Synonyms: manR, yjdC

Genomic Context

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List of homologs in different organisms, belongs to COG3711 (Galperin et al., 2021)

Gene

Coordinates: 1,270,631 → 1,272,577

The protein

Catalyzed reaction/ biological activity

• D-mannose + N^π-phospho-L-histidyl-[protein] --> D-mannose 6-phosphate + L-histidyl-[protein] (according to UniProt)

Protein family

• PRD-containing transcription factors

Structure

• O31644 (AlphaFold)

Modification

• phosphorylation by ManP inactivates ManR (in the absence of mannose), phosphorylation by HPr stimluates ManR PubMed

Expression and Regulation

Operons

• Genes: manR

  Description: PubMed

  Regulation

  • induced by mannose (ManR) PubMed

  Regulatory mechanism

  • ManR: activation, in the presence of mannose and absence of glucose PubMed, in manR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A269 (yjdC::erm), available at the NBRP B. subtilis, Japan
• BKE12000 (ΔmanR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGTTTTTCTATCCTTC,  downstream forward: _UP4_TAAAAGCAGGGATTATTCCT
• BKK12000 (ΔmanR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGTTTTTCTATCCTTC,  downstream forward: _UP4_TAAAAGCAGGGATTATTCCT

References

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