CwlJ

• Description: spore coat protein, cell wall hydrolase
  
  

Gene name	cwlJ
Synonyms	ycbQ
Essential	no
Product	spore coat protein, cell wall hydrolase
Function	spore germination
Gene expression levels in SubtiExpress: cwlJ
MW, pI	16 kDa, 9.354
Gene length, protein length	426 bp, 142 aa
Immediate neighbours	ycbP, ycbR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall/ other, sporulation proteins, germination

This gene is a member of the following regulons

SigE regulon, SigK regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU02600

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: cwlJ family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • outer edge of the spore cortex PubMed
  • inner spore coat, localization depends on SafA PubMed

Database entries

• Structure:

• UniProt: P42249

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cwlJ PubMed

• Expression browser: cwlJ PubMed

• Sigma factor: SigE PubMed, SigK PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigE, SigK, SpoIIID) PubMed

• Regulatory mechanism:
  • SpoIIID: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1G19 ( cwlJ::tet), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Peter T McKenney, Adam Driks, Patrick Eichenberger
The Bacillus subtilis endospore: assembly and functions of the multilayered coat.
Nat Rev Microbiol: 2013, 11(1);33-44
[PubMed:23202530] [WorldCat.org] [DOI] (I p)