UvrB

• Description: excinuclease ABC (subunit B)
  
  

Gene name	uvrB
Synonyms	dinA, uvrA
Essential	no
Product	excinuclease ABC (subunit B)
Function	DNA repair after UV damage
Gene expression levels in SubtiExpress: uvrB
Interactions involving this protein in SubtInteract: UvrB
MW, pI	76 kDa, 5.26
Gene length, protein length	1983 bp, 661 aa
Immediate neighbours	uvrA, csbA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU35170

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: uvrB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • UvrA-UvrB PubMed
  • UvrB-UvrC PubMed
  • UvrB interacts sequentially with UvrA and UvrC, a complex of the three proteins was never observed PubMed
  • UvrB-PcrA PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2NMV (bound to fluorescein-adducted DNA); 2D7D ternary complex involving UvrB* (a C-terminal truncation of full-length UvrB), a polythymine trinucleotide and ADP PubMed

• UniProt: P37954

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: uvrB-uvrA (according to DBTBS)

• Expression browser: uvrB PubMed

• Sigma factor:

• Regulation:
  • induced upon DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1175 (del uvrAB::ermC) (available in the Stülke lab)

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

James J Truglio, Deborah L Croteau, Bennett Van Houten, Caroline Kisker
Prokaryotic nucleotide excision repair: the UvrABC system.
Chem Rev: 2006, 106(2);233-52
[PubMed:16464004] [WorldCat.org] [DOI] (P p)

Bennett Van Houten, Deborah L Croteau, Matthew J DellaVecchia, Hong Wang, Caroline Kisker
'Close-fitting sleeves': DNA damage recognition by the UvrABC nuclease system.
Mutat Res: 2005, 577(1-2);92-117
[PubMed:15927210] [WorldCat.org] [DOI] (P p)

A Sancar
Mechanisms of DNA excision repair.
Science: 1994, 266(5193);1954-6
[PubMed:7801120] [WorldCat.org] [DOI] (P p)