uvrC

168

excinuclease ABC (subunit C), required for transcription-dependent asymmetry in mutation rates of genes in the two orientations

Locus: BSU_28490

Molecular weight: 69.28 kDa

Isoelectric point: 9.5

Protein length: 590 aa

Gene length: 1773 bp

Function: DNA repair after UV damage

Product: excinuclease ABC (subunit C)

Essential: no

E.C.: null

Synonyms: uvrC, uvrB

Genomic Context

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List of homologs in different organisms, belongs to COG0322 (Galperin et al., 2021)

Gene

Coordinates: 2,911,116  2,912,888

The protein

Protein family

• uvrC family (single member, according to UniProt)

Domains

• GIY-YIG-domain (aa 14-91) (according to UniProt)
• UVR domain (aa 196-231) (according to UniProt)

Structure

• 3C65 (PDB) (5' endonuclease domain, Geobacillus stearothermophilus)
• P14951 (AlphaFold)

Expression and Regulation

Operons

• Genes: uvrC

  Description: PubMed

  Regulation

  • induced upon DNA damage (LexA) PubMed

  Regulatory mechanism

  • LexA: repression, PubMed], in lexA regulon

Biological materials

Mutant

• GP3542 (ΔuvrC::kan), available in Jörg Stülke's lab
• BKE28490 (uvrC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGTTACCCTTCCTTAT,  downstream forward: _UP4_TAATGTTGTCCTTTTAAATA
• BKK28490 (uvrC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGTTACCCTTCCTTAT,  downstream forward: _UP4_TAATGTTGTCCTTTTAAATA

References

Reviews

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Original Publications

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