Tkt

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  • Description: transketolase

Gene name tkt
Synonyms tktA
Essential no
Product transketolase
Function pentose phosphate pathway
Gene expression levels in SubtiExpress: tkt
Interactions involving this protein in SubtInteract: Tkt
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 72 kDa, 4.803
Gene length, protein length 2001 bp, 667 aa
Immediate neighbours ynzC, sirA
Sequences Protein DNA DNA_with_flanks
Genetic context
Tkt context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Tkt expression.png















Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

  • Locus tag: BSU17890

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate (according to Swiss-Prot)
  • Protein family: transketolase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on ser/ thr/ tyr PubMed
  • Cofactor(s): thiamine pyrophosphate
  • Effectors of protein activity:

Database entries

  • Structure: [3K95], complex with thiamine diphosphate, from B. anthracis
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: BS4530 (aphA3), available in Stülke lab
  • Expression vector:
    • pGP94 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Stülke lab
    • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP820, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:
  • FLAG-tag construct: GP1406 (spc, based on pGP1331), available in the Stülke lab

Labs working on this gene/protein

Your additional remarks

References

Reviews

G Schenk, R G Duggleby, P F Nixon
Properties and functions of the thiamin diphosphate dependent enzyme transketolase.
Int J Biochem Cell Biol: 1998, 30(12);1297-318
[PubMed:9924800] [WorldCat.org] [DOI] (P p)

Original publications