sirA

168

Sporulation protein, inhibits DNA replication initiation in cells committed to Sporulation, facilitates capture of oriC in the forespore

Locus: BSU_17900

Molecular weight: 18.00 kDa

Isoelectric point: 7.35

Protein length: 148 aa

Gene length: 447 bp

Function: control of chromosome copy number

Product: inhibitor of DNA replication

Essential: no

Synonyms: sirA, yneE, yoxF

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 1,922,017  1,922,463

Phenotypes of a mutant

• over-replication during Sporulation PubMed
• defective in oriC segregation during Sporulation PubMed

The protein

Catalyzed reaction/ biological activity

• interacts with domain I of DnaA and displaces it from the replication origin, resulting in inhibition of replication PubMed
• interacts with domain III of DnaA to facilitate ParA-dependent oriC capture in the forespore PubMed
• interaction with DnaA prevents the DnaA-DnaD interaction PubMed

Structure

• 4TPS (PDB) (in complex with N-terminal domain of DnaA) PubMed
• P45707 (AlphaFold)

Localization

• accumulates at the replisome in sporulating cells (perhaps in DnaA-dependent manner) PubMed

Expression and Regulation

Operons

• Genes: sirA-yneF

  Description: PubMed

  Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed

  Regulatory mechanism

  • Spo0A: activation, PubMed, in spo0A regulon

Biological materials

Mutant

• MGNA-B389 (yneE::erm), available at the NBRP B. subtilis, Japan
• BKE17900 (sirA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTTTCATCCCCTTTTC,  downstream forward: _UP4_TAAAACCGAAGAAAAAGAGA
• BKK17900 (sirA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTTTCATCCCCTTTTC,  downstream forward: _UP4_TAAAACCGAAGAAAAAGAGA

References

Reviews

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Original Publications

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