FadE

• Description: acyl-CoA dehydrogenase
  
  

Gene name	fadE
Synonyms	yusJ
Essential	no
Product	acyl-CoA dehydrogenase
Function	fatty acid degradation
Gene expression levels in SubtiExpress: fadE
Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation
MW, pI	65 kDa, 5.146
Gene length, protein length	1782 bp, 594 aa
Immediate neighbours	yusI, fadA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed 500px

Categories containing this gene/protein

utilization of lipids, membrane proteins

This gene is a member of the following regulons

CcpA regulon, FadR regulon, SdpR regulon

The gene

Basic information

• Locus tag: BSU32820

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acyl-CoA + ETF = 2,3-dehydroacyl-CoA + reduced ETF (according to Swiss-Prot)

• Protein family: acyl-CoA dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s): YngJ, MmgC, AcdA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane PubMed

Database entries

• Structure: 2Z1Q (from Thermus thermophilus hb8, 52% identity, 68% similarity)

• UniProt: O32176

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • fadM-fadN-fadA-fadE PubMed
  • fadN-fadA-fadE PubMed

• Sigma factor:
  • fadN: SigA PubMed

• Regulation:
  • fadN: induced by long-chain fatty acids (FadR) PubMed
  • fadN: subject to carbon catabolite repression (CcpA-HPr(Ser-P) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed
  • SdpR: transcription activation PubMed
  • CcpA-HPr(Ser-P): transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Imke G de Jong, Jan-Willem Veening, Oscar P Kuipers
Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation.
Environ Microbiol: 2012, 14(12);3110-21
[PubMed:23033921] [WorldCat.org] [DOI] (I p)

Shigeo Tojo, Takenori Satomura, Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Catabolite repression of the Bacillus subtilis FadR regulon, which is involved in fatty acid catabolism.
J Bacteriol: 2011, 193(10);2388-95
[PubMed:21398533] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)

José E González-Pastor, Errett C Hobbs, Richard Losick
Cannibalism by sporulating bacteria.
Science: 2003, 301(5632);510-3
[PubMed:12817086] [WorldCat.org] [DOI] (I p)