IolB

• Description: formation of 2-deoxy-5-keto-gluconic acid (4th reaction)
  
  

Gene name	iolB
Synonyms	yxdB
Essential	no
Product	formation of 2-deoxy-5-keto-gluconic acid (4th reaction)
Function	myo-inositol catabolism
Gene expression levels in SubtiExpress: iolB
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	30 kDa, 4.77
Gene length, protein length	813 bp, 271 aa
Immediate neighbours	iolC, iolA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, IolR regulon

The gene

Basic information

• Locus tag: BSU39750

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5-deoxy-glucuronic acid = 5-dehydro-2-deoxy-D-gluconic acid (according to Swiss-Prot)

• Protein family: isomerase iolB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2QJV (from Salmonella typhimurium, 49% identity, 65% similarity)

• UniProt: P42413

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: iolA-iolB-iolC-iolD-iolE-iolF-iolG-iolH-iolI-iolJ PubMed

• Expression browser: iolB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by inositol (IolR) PubMed

• Regulatory mechanism:
  • IolR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Yasutaro Fujita, University of Fukuyama, Japan
• Ken-ichi Yoshida, Kobe University, Japan