TenI

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  • Description: thiazole tautomerase

Gene name tenI
Synonyms
Essential no
Product thiazole tautomerase
Function biosynthesis of thiamine
Gene expression levels in SubtiExpress: tenI
Metabolic function and regulation of this protein in SubtiPathways:
Thiamin
MW, pI 22 kDa, 8.132
Gene length, protein length 615 bp, 205 aa
Immediate neighbours tenA, thiO
Sequences Protein DNA DNA_with_flanks
Genetic context
TenI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TenI expression.png



















Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

Thi-box

The gene

Basic information

  • Locus tag: BSU11660

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: TMP-PPase family (according to Swiss-Prot)
  • Paralogous protein(s): ThiE

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Tadhg P Begley, Steven E Ealick, Fred W McLafferty
Thiamin biosynthesis: still yielding fascinating biological chemistry.
Biochem Soc Trans: 2012, 40(3);555-60
[PubMed:22616866] [WorldCat.org] [DOI] (I p)

T P Begley, D M Downs, S E Ealick, F W McLafferty, A P Van Loon, S Taylor, N Campobasso, H J Chiu, C Kinsland, J J Reddick, J Xi
Thiamin biosynthesis in prokaryotes.
Arch Microbiol: 1999, 171(5);293-300
[PubMed:10382260] [WorldCat.org] [DOI] (P p)

Original publications

Amrita B Hazra, Ying Han, Abhishek Chatterjee, Yang Zhang, Rung-Yi Lai, Steven E Ealick, Tadhg P Begley
A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase.
J Am Chem Soc: 2011, 133(24);9311-9
[PubMed:21534620] [WorldCat.org] [DOI] (I p)

Angela V Toms, Amy L Haas, Joo-Heon Park, Tadhg P Begley, Steven E Ealick
Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II.
Biochemistry: 2005, 44(7);2319-29
[PubMed:15709744] [WorldCat.org] [DOI] (P p)

A S Pang, S Nathoo, S L Wong
Cloning and characterization of a pair of novel genes that regulate production of extracellular enzymes in Bacillus subtilis.
J Bacteriol: 1991, 173(1);46-54
[PubMed:1898926] [WorldCat.org] [DOI] (P p)