ThiE

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  • Description: thiamine-phosphate pyrophosphorylase

Gene name thiE
Synonyms thiC, ipa-26d, ywbK
Essential no
Product thiamine-phosphate pyrophosphorylase
Function biosynthesis of thiamine
by 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate
to yield thiamine phosphate
Gene expression levels in SubtiExpress: thiE
Metabolic function and regulation of this protein in SubtiPathways:
Thiamin
MW, pI 23 kDa, 5.058
Gene length, protein length 666 bp, 222 aa
Immediate neighbours ywbL, thiM
Sequences Protein DNA DNA_with_flanks
Genetic context
ThiE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ThiE expression.png






























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU38290

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate (according to Swiss-Prot)
  • Protein family: TMP-PPase family (according to Swiss-Prot)
  • Paralogous protein(s): TenI

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications

Y Zhang, S V Taylor, H J Chiu, T P Begley
Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.
J Bacteriol: 1997, 179(9);3030-5
[PubMed:9139923] [WorldCat.org] [DOI] (P p)