ThiE

• Description: thiamine-phosphate pyrophosphorylase
  
  

Gene name	thiE
Synonyms	thiC, ipa-26d, ywbK
Essential	no
Product	thiamine-phosphate pyrophosphorylase
Function	biosynthesis of thiamine by 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate to yield thiamine phosphate
Gene expression levels in SubtiExpress: thiE
Metabolic function and regulation of this protein in SubtiPathways: Thiamin
MW, pI	23 kDa, 5.058
Gene length, protein length	666 bp, 222 aa
Immediate neighbours	ywbL, thiM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

The gene

Basic information

• Locus tag: BSU38290

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate (according to Swiss-Prot)

• Protein family: TMP-PPase family (according to Swiss-Prot)

• Paralogous protein(s): TenI

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1G6C

• UniProt: P39594

• KEGG entry: [3]

• E.C. number: 2.5.1.3

Expression and regulation

• Operon: ywbI-thiM-thiE PubMed

• Expression browser: thiE PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications

Y Zhang, S V Taylor, H J Chiu, T P Begley
Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.
J Bacteriol: 1997, 179(9);3030-5
[PubMed:9139923] [WorldCat.org] [DOI] (P p)