PhrF

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Gene name phrF
Synonyms ywhI
Essential no
Product phosphatase (RapF) regulator
Function control of ComA activity
Gene expression levels in SubtiExpress: phrF
Interactions involving this protein in SubtInteract: PhrF
MW, pI 4 kDa, 10.237
Gene length, protein length 117 bp, 39 aa
Immediate neighbours rapF, ywhH
Sequences Protein DNA DNA_with_flanks
Genetic context
PhrF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PhrF expression.png
























Categories containing this gene/protein

genetic competence, transcription factors and their control, quorum sensing, short peptides

This gene is a member of the following regulons

ComA regulon, SigH regulon

The gene

Basic information

  • Locus tag: BSU37470

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binds to RapF, this results in the inability of RapF to interact with ComA PubMed
  • Protein family: phr family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • PhrF accumulates at high cell density
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Melinda D Baker, Matthew B Neiditch
Structural basis of response regulator inhibition by a bacterial anti-activator protein.
PLoS Biol: 2011, 9(12);e1001226
[PubMed:22215984] [WorldCat.org] [DOI] (I p)

Jennifer M Auchtung, Catherine A Lee, Alan D Grossman
Modulation of the ComA-dependent quorum response in Bacillus subtilis by multiple Rap proteins and Phr peptides.
J Bacteriol: 2006, 188(14);5273-85
[PubMed:16816200] [WorldCat.org] [DOI] (P p)

Natalia Comella, Alan D Grossman
Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis.
Mol Microbiol: 2005, 57(4);1159-74
[PubMed:16091051] [WorldCat.org] [DOI] (P p)

Cristina Bongiorni, Shu Ishikawa, Sophie Stephenson, Naotake Ogasawara, Marta Perego
Synergistic regulation of competence development in Bacillus subtilis by two Rap-Phr systems.
J Bacteriol: 2005, 187(13);4353-61
[PubMed:15968044] [WorldCat.org] [DOI] (P p)

R S McQuade, N Comella, A D Grossman
Control of a family of phosphatase regulatory genes (phr) by the alternate sigma factor sigma-H of Bacillus subtilis.
J Bacteriol: 2001, 183(16);4905-9
[PubMed:11466295] [WorldCat.org] [DOI] (P p)