rapF

168

response regulator aspartate phosphatase, anti-activator protein, controls ComA activity

Locus: BSU_37460

Molecular weight: 45.45 kDa

Isoelectric point: 5.18

Protein length: 381 aa

Gene length: 1146 bp

Function: control of ComA activity

Product: response regulator aspartate phosphatase

Essential: no

Synonyms: rapF, ywhJ

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 3,846,001  3,847,146

The protein

Catalyzed reaction/ biological activity

• control of ComA activity PubMed

Protein family

• RAP family (according to UniProt)

Domains

• six tetratrichopeptide repeats (according to UniProt)

Structure

• 4I9E (PDB) PubMed
• 3ULQ (PDB) (structure of the complex between the DNA-binding C-terminal domain of ComA with RapF) PubMed
• P71002 (AlphaFold)

Effectors of protein activity

• binding of PhrF to RapF results in a conformational change of RapF and prevents it from binding ComA PubMed

Expression and Regulation

Operons

• Genes: rapF-phrF

  Description: PubMed

  Regulation

  • PhrF accumulates at high cell density

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • ComA: activation, PubMed, in comA regulon

  Sigma factors

  • SigH: sigma factor, PubMed, in sigH regulon

Biological materials

Mutant

• MGNA-A035 (rapF::erm), available at the NBRP B. subtilis, Japan
• BKE37460 (rapF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTTGCAAATCCCTCCC,  downstream forward: _UP4_CTTATTCAAGGAGGAGTGAG
• BKK37460 (rapF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTTGCAAATCCCTCCC,  downstream forward: _UP4_CTTATTCAAGGAGGAGTGAG

References

Reviews

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Original Publications

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