YvcJ

• Description: GTPase, nucleotide-binding protein
  
  

Gene name	yvcJ
Synonyms
Essential	no
Product	GTPase
Function	required for the expression of late competence genes
Gene expression levels in SubtiExpress: yvcJ
MW, pI	33 kDa, 6.702
Gene length, protein length	885 bp, 295 aa
Immediate neighbours	yvcK, yvcI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence

The gene

Basic information

• Locus tag: BSU34770

Phenotypes of a mutant

• reduced genetic competence PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds and hydrolyzes nucleotides, required for the expression of late competence genes (comK, comS) PubMed

• Protein family: UPF0042 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • can be localized in the cell either as a helical-like pattern or as foci close to the poles and the septa depending on growth phase and on growth medium PubMed

Database entries

• Structure:

• UniProt: O06973

• KEGG entry: [2]

• E.C. number:

Additional information

• Information on the E. coli homolog

Expression and regulation

• Operon: yvcI-yvcJ-yvcK-yvcL-crh-yvcN PubMed

• Expression browser: yvcJ PubMed

• Sigma factor: SigA PubMed

• Regulation: very weak stimuation of expression by citrate and succinate PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A885 ( yvcJ::cat), PubMed, available at BGSC

• Expression vector: pGP735 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

• Antibody:

Labs working on this gene/protein

Anne Galinier, University of Marseille, France

References

Publications on the E. coli homolog

Falk Kalamorz, Birte Reichenbach, Walter März, Bodo Rak, Boris Görke
Feedback control of glucosamine-6-phosphate synthase GlmS expression depends on the small RNA GlmZ and involves the novel protein YhbJ in Escherichia coli.
Mol Microbiol: 2007, 65(6);1518-33
[PubMed:17824929] [WorldCat.org] [DOI] (P p)