GlsA

• Description: glutaminase, low affinity for glutamine
  
  

Gene name	glsA
Synonyms	ybgJ
Essential	no
Product	glutaminase
Function	glutamine degradation
Gene expression levels in SubtiExpress: glsA
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	36 kDa, 5.315
Gene length, protein length	981 bp, 327 aa
Immediate neighbours	glnT, glnK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

GlnL regulon

The gene

Basic information

• Locus tag: BSU02430

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: l-glutamine + H₂O = L-glutamate + NH₃ (according to Swiss-Prot)

• Protein family: glutaminase family (according to Swiss-Prot)

• Paralogous protein(s): YlaM

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3BRM PubMed

• UniProt: O31465

• KEGG entry: [3]

• E.C. number: 3.5.1.2

Expression and regulation

• Operon: glsA-glnT PubMed

• Expression browser: glsA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the presence of glutamine (GlnL) PubMed

• Regulatory mechanism:
  • GlnL: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: