MenD

• Description: 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / 2-oxoglutarate decarboxylase
  
  

Gene name	menD
Synonyms
Essential	yes PubMed
Product	2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / 2-oxoglutarate decarboxylase
Function	biosynthesis of menaquinone
Gene expression levels in SubtiExpress: menD
Metabolic function and regulation of this protein in SubtiPathways: Menaquinone
MW, pI	63 kDa, 5.655
Gene length, protein length	1740 bp, 580 aa
Immediate neighbours	ytxM, menF
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, essential genes

The gene

Basic information

• Locus tag: BSU30820

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO₂ (according to Swiss-Prot)

• Protein family: MenD subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): thiamine pyrophosphate, divalent metal ion such as Mn(2+)) PubMed

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2X7J PubMed

• UniProt: P23970

• KEGG entry: [3]

• E.C. number: 2.2.1.9

Expression and regulation

• Operon: menF-menD-ytxM-menB-menE-menC PubMed

• Expression browser: menD PubMed

• Sigma factor: SigA: PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alice Dawson, Minjiao Chen, Paul K Fyfe, Zhihong Guo, William N Hunter
Structure and reactivity of Bacillus subtilis MenD catalyzing the first committed step in menaquinone biosynthesis.
J Mol Biol: 2010, 401(2);253-64
[PubMed:20600129] [WorldCat.org] [DOI] (I p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)

X Qin, H W Taber
Transcriptional regulation of the Bacillus subtilis menp1 promoter.
J Bacteriol: 1996, 178(3);705-13
[PubMed:8550504] [WorldCat.org] [DOI] (P p)

J R Driscoll, H W Taber
Sequence organization and regulation of the Bacillus subtilis menBE operon.
J Bacteriol: 1992, 174(15);5063-71
[PubMed:1629163] [WorldCat.org] [DOI] (P p)