AnsR

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  • Description: transcription repressor of the ansA-ansB operon

Gene name ansR
Synonyms
Essential no
Product transcriptional regulator (Xre family)
Function negative regulation of the ansA-ansB operon
Gene expression levels in SubtiExpress: ansR
Metabolic function and regulation of this protein in SubtiPathways:
Asp, Asn
MW, pI 13 kDa, 4.807
Gene length, protein length 348 bp, 116 aa
Immediate neighbours ansA, yqxK
Sequences Protein DNA Advanced_DNA
Genetic context
AnsR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AnsR expression.png




























Categories containing this gene/protein

utilization of amino acids, transcription factors and their control

This gene is a member of the following regulons

AnsR regulon

The AnsR regulon:

The gene

Basic information

  • Locus tag: BSU23590

Phenotypes of a mutant

  • the ansR mutation allows glutamate utilization of a rocG gudB mutant PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • induced in the presence of asparagine (AnsR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1152 (del tet) available in the Stülke lab
  • Expression vector:
    • expression of native ansR in B. subtilis: pGP873 (in pBQ200), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Lope A Flórez, Katrin Gunka, Rafael Polanía, Stefan Tholen, Jörg Stülke
SPABBATS: A pathway-discovery method based on Boolean satisfiability that facilitates the characterization of suppressor mutants.
BMC Syst Biol: 2011, 5;5
[PubMed:21219666] [WorldCat.org] [DOI] (I e)

Susan H Fisher, Lewis V Wray
Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.
J Bacteriol: 2002, 184(8);2148-54
[PubMed:11914346] [WorldCat.org] [DOI] (P p)

D Sun, P Setlow
Cloning and nucleotide sequence of the Bacillus subtilis ansR gene, which encodes a repressor of the ans operon coding for L-asparaginase and L-aspartase.
J Bacteriol: 1993, 175(9);2501-6
[PubMed:8478318] [WorldCat.org] [DOI] (P p)