YlbF

• Description: antagonist of biofilm repression by SinR, control of the phosphorelay
  
  

Gene name	ylbF
Synonyms
Essential	no
Product	antagonist of biofilm repression by SinR, control of ComK stability
Function	regulation of biofilm formation and the phosphorelay
Gene expression levels in SubtiExpress: ylbF
Interactions involving this protein in SubtInteract: YlbF
MW, pI	16 kDa, 5.012
Gene length, protein length	447 bp, 149 aa
Immediate neighbours	ylbE, ylbG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, phosphorelay, biofilm formation

The gene

Basic information

• Locus tag: BSU14990

Phenotypes of a mutant

• strongly reduced genetic competence, strongly reduced sporulation PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • YmcA-YlbF-YaaT, this complex stimulates Spo0A phosphorylation in the phosphorelay PubMed

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34412

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ylbF PubMed

• Sigma factor:

• Regulation:
  • expressed during growth and the transition phase, expression is erduced in stationary phase (Spo0A-P) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Valerie J Carabetta, Andrew W Tanner, Todd M Greco, Melissa Defrancesco, Ileana M Cristea, David Dubnau
A complex of YlbF, YmcA and YaaT regulates sporulation, competence and biofilm formation by accelerating the phosphorylation of Spo0A.
Mol Microbiol: 2013, 88(2);283-300
[PubMed:23490197] [WorldCat.org] [DOI] (I p)

Daniel B Kearns, Frances Chu, Steven S Branda, Roberto Kolter, Richard Losick
A master regulator for biofilm formation by Bacillus subtilis.
Mol Microbiol: 2005, 55(3);739-49
[PubMed:15661000] [WorldCat.org] [DOI] (P p)

Steven S Branda, José Eduardo González-Pastor, Etienne Dervyn, S Dusko Ehrlich, Richard Losick, Roberto Kolter
Genes involved in formation of structured multicellular communities by Bacillus subtilis.
J Bacteriol: 2004, 186(12);3970-9
[PubMed:15175311] [WorldCat.org] [DOI] (P p)

P Tortosa, M Albano, D Dubnau
Characterization of ylbF, a new gene involved in competence development and sporulation in Bacillus subtilis.
Mol Microbiol: 2000, 35(5);1110-9
[PubMed:10712692] [WorldCat.org] [DOI] (P p)