MtlA

• Description: trigger enzyme: mannitol-specific phosphotransferase system, EIICB of the PTS
  
  

Gene name	mtlA
Synonyms
Essential	no
Product	trigger enzyme: mannitol-specific phosphotransferase system, EIICB
Function	mannitol uptake and phosphorylation, control of MtlR activity
Gene expression levels in SubtiExpress: mtlA
Interactions involving this protein in SubtInteract: MtlA
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	50 kDa, 8.814
Gene length, protein length	1434 bp, 478 aa
Immediate neighbours	ycnL, mtlF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, transcription factors and their control, trigger enzyme, membrane proteins, phosphoproteins

This gene is a member of the following regulons

MtlR regulon

The gene

Basic information

• Locus tag: BSU03981

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

• Protein family: PTS permease, fructose/ mannitol permease (Fru) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-559 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • MtlA-MtlR PubMed (the non-phosphorylated IIB domain of MtlA interacts with the two C-terminal EIIB (Gat)-like and EIIA (Mtl)-like domains of MtlR) PubMed

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1A3A (from Escherichia coli k12 mutant, 42% identity, 66% similarity) PubMed

• UniProt: P42956

• KEGG entry: [3]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: mtlA-mtlF-mtlD PubMed

• Expression browser: mtlA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by mannitol (MtlR) PubMed
  • repressed by glucose (MtlR) PubMed

• Regulatory mechanism:
  • MtlR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Houda Bouraoui, Magali Ventroux, Marie-Françoise Noirot-Gros, Josef Deutscher, Philippe Joyet
Membrane sequestration by the EIIB domain of the mannitol permease MtlA activates the Bacillus subtilis mtl operon regulator MtlR.
Mol Microbiol: 2013, 87(4);789-801
[PubMed:23279188] [WorldCat.org] [DOI] (I p)