mtlA

168

mannitol-specific permease of the phosphotransferase system, EIICB of the PTS, Trigger enzyme, control of MtlR activity

Locus: BSU_03981

Molecular weight: 50.00 kDa

Isoelectric point: 8.814

Protein length: 478 aa

Gene length: 1437 bp

Function: mannitol uptake and phosphorylation, control of MtlR activity

Product: mannitol-specific PTS, EIICB

Essential: no

E.C.: 2.7.1.69

Synonyms: mtlA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2213 (Galperin et al., 2021)

Gene

Coordinates: 449,724  451,160

The protein

Catalyzed reaction/ biological activity

• D-mannitol + N^π-phospho-L-histidyl-[protein] --> D-mannitol 1-phosphate + L-histidyl-[protein] (according to UniProt)

Protein family

• PTS permease, fructose/ mannitol family PubMed

Domains

• 2 PTS EIIC domains type-2 (aa 18-347, aa 390-478) (according to UniProt)

Structure

• 1A3A (PDB) (from Escherichia coli k12 mutant, 42% identity, 66% similarity)\tPubMed
• P42956 (AlphaFold)

Modification

• phosphorylation on Ser-559 PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: mtlA-mtlF-mtlD

  Description: PubMed

  Regulation

  • induced by mannitol (MtlR) PubMed

  Regulatory mechanism

  • MtlR: activation, PubMed, in mtlR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • An antisense RNA is predicted for mtlD PubMed

Biological materials

Mutant

• MGNA-C018 (mtlA::erm), available at the NBRP B. subtilis, Japan
• BKE03981 (mtlA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATATAAACCCTCCCTGT,  downstream forward: _UP4_TAATCTTATAGAAAGAGAGT
• BKK03981 (mtlA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATATAAACCCTCCCTGT,  downstream forward: _UP4_TAATCTTATAGAAAGAGAGT

References

Reviews

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Original Publications

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