Epr

• Description: minor extracellular serine protease, involved in control of swarming motility PubMed
  
  

Gene name	epr
Synonyms	ipa-15r
Essential	no
Product	minor extracellular serine protease
Function	protein degradation
Gene expression levels in SubtiExpress: epr
MW, pI	69 kDa, 9.875
Gene length, protein length	1935 bp, 645 aa
Immediate neighbours	ywbA, sacX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of nitrogen sources other than amino acids, proteolysis, motility and chemotaxis

This gene is a member of the following regulons

DegU regulon, ScoC regulon, SigD regulon, SinR regulon

The gene

Basic information

• Locus tag: BSU38400

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: peptidase S8 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: P16396

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: epr PubMed

• Expression browser: epr PubMed

• Sigma factor: SigD PubMed

• Regulation:
  • repressed by SinR PubMed
  • repressed during exponential growth (ScoC) PubMed

• Regulatory mechanism:
  • activation by DegU, not activated by DegU-P PubMed
  • SinR: transcription repression PubMed
  • ScoC: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A1021 ( epr::tet), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)