ThiS

• Description: sulfur carrier protein, hydroxyethylthiazole phosphate biosynthesis
  
  

Gene name	thiS
Synonyms	yjbS
Essential	no
Product	sulfur carrier protein
Function	biosynthesis of thiamine
Gene expression levels in SubtiExpress: thiS
Interactions involving this protein in SubtInteract: ThiS
Metabolic function and regulation of this protein in SubtiPathways: Thiamin
MW, pI	7 kDa, 5.501
Gene length, protein length	198 bp, 66 aa
Immediate neighbours	thiO, thiG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

Thi-box

The gene

Basic information

• Locus tag: BSU11680

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: ThiS-ThiG PubMed

• Localization:

Database entries

• Structure: 1TYG (complex with ThiG) PubMed

• UniProt: O31617

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: tenA-tenI-thiO-thiS-thiG-thiF-thiD PubMed

• Expression browser: thiS PubMed

• Sigma factor:

• Regulation:
  • repressed by thiamine (Thi-box) PubMed

• Regulatory mechanism:
  • Thi-box: RNA switch PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

T P Begley, D M Downs, S E Ealick, F W McLafferty, A P Van Loon, S Taylor, N Campobasso, H J Chiu, C Kinsland, J J Reddick, J Xi
Thiamin biosynthesis in prokaryotes.
Arch Microbiol: 1999, 171(5);293-300
[PubMed:10382260] [WorldCat.org] [DOI] (P p)

Original publications

Ethan C Settembre, Pieter C Dorrestein, Huili Zhai, Abhishek Chatterjee, Fred W McLafferty, Tadhg P Begley, Steven E Ealick
Thiamin biosynthesis in Bacillus subtilis: structure of the thiazole synthase/sulfur carrier protein complex.
Biochemistry: 2004, 43(37);11647-57
[PubMed:15362849] [WorldCat.org] [DOI] (P p)

Joo-Heon Park, Pieter C Dorrestein, Huili Zhai, Cynthia Kinsland, Fred W McLafferty, Tadhg P Begley
Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).
Biochemistry: 2003, 42(42);12430-8
[PubMed:14567704] [WorldCat.org] [DOI] (P p)