ThiS

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  • Description: sulfur carrier protein, hydroxyethylthiazole phosphate biosynthesis

Gene name thiS
Synonyms yjbS
Essential no
Product sulfur carrier protein
Function biosynthesis of thiamine
Gene expression levels in SubtiExpress: thiS
Interactions involving this protein in SubtInteract: ThiS
Metabolic function and regulation of this protein in SubtiPathways:
Thiamin
MW, pI 7 kDa, 5.501
Gene length, protein length 198 bp, 66 aa
Immediate neighbours thiO, thiG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ThiS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ThiS expression.png




























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

Thi-box

The gene

Basic information

  • Locus tag: BSU11680

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

T P Begley, D M Downs, S E Ealick, F W McLafferty, A P Van Loon, S Taylor, N Campobasso, H J Chiu, C Kinsland, J J Reddick, J Xi
Thiamin biosynthesis in prokaryotes.
Arch Microbiol: 1999, 171(5);293-300
[PubMed:10382260] [WorldCat.org] [DOI] (P p)

Original publications

Ethan C Settembre, Pieter C Dorrestein, Huili Zhai, Abhishek Chatterjee, Fred W McLafferty, Tadhg P Begley, Steven E Ealick
Thiamin biosynthesis in Bacillus subtilis: structure of the thiazole synthase/sulfur carrier protein complex.
Biochemistry: 2004, 43(37);11647-57
[PubMed:15362849] [WorldCat.org] [DOI] (P p)

Joo-Heon Park, Pieter C Dorrestein, Huili Zhai, Cynthia Kinsland, Fred W McLafferty, Tadhg P Begley
Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).
Biochemistry: 2003, 42(42);12430-8
[PubMed:14567704] [WorldCat.org] [DOI] (P p)