Pgk

From SubtiWiki
Revision as of 16:02, 7 August 2012 by 134.76.70.252 (talk)
Jump to: navigation, search
Gene name pgk
Synonyms
Essential No
Product phosphoglycerate kinase
Function enzyme in glycolysis/ gluconeogenesis
Gene expression levels in SubtiExpress: pgk
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 42,0 kDa, 4.77
Gene length, protein length 1182 bp, 394 amino acids
Immediate neighbours tpi, gapA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Pgk context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Pgk expression.png




























Categories containing this gene/protein

ATP synthesis, carbon core metabolism, phosphoproteins, universally conserved proteins

This gene is a member of the following regulons

CggR regulon

The gene

Basic information

  • Locus tag: BSU33930

Phenotypes of a mutant

  • no growth on LB medium, the mutant requires glucose and malate for growth
  • essential according to Kobayashi et al. PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + 3-phospho-D-glycerate = ADP + 1,3-bisphosphoglycerate (according to Swiss-Prot)
  • Protein family: phosphoglycerate kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: Two Substrate Reversible Michaelis-Menten PubMed
  • Domains:
    • nucleotide binding domain (ATP) (350–353)
    • 2x substrate binding domain (21–23), (59–62)
  • Modification: phosphorylation on Ser-183 AND Thr-299 PubMed, PubMed
  • Cofactor(s): Mg2+ or Mn2+ PubMed
  • Effectors of protein activity:
    • Inhibited by Co2+, NDP and NMP PubMed

Database entries

  • Structure: 1PHP (from Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

  • extensive information on the structure and enzymatic properties of Pgk can be found at Proteopedia

Expression and regulation

  • Regulatory mechanism: transcription repression by CggR PubMed
  • Additional information:

Biological materials

  • Mutant:
    • GP699 (pgk::cat), available in Stülke lab
    • GP707 (pgk::erm), available in Stülke lab
  • Expression vector:
    • pGP1102 (N-terminal His-tag, in pWH844), available in Stülke lab
    • pGP95 (N-terminal Strep-tag, in pGP172), available in Stülke lab
    • pGP91 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Stülke lab
    • pGP1513 (expression in B. subtilis, in pBQ200), available in Stülke lab
  • lacZ fusion: pGP514 (in pAC6), a series of promoter deletion variants is also available in pAC6, available in Stülke lab
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References