MoaE

• Description: molybdopterin synthase (large subunit), catalyses the transfer of sulfide from MoaD thiocarboxylate
  
  

Gene name	moaE
Synonyms
Essential	no
Product	molybdopterin synthase (large subunit)
Function	nitrate respiration
MW, pI	17 kDa, 4.746
Gene length, protein length	471 bp, 157 aa
Immediate neighbours	mobB, moaD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

The gene

Basic information

• Locus tag: BSU14300

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: catalyses the transfer of sulfide from MoaD thiocarboxylate

• Protein family: moaE family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:
  • 1NVI (the MoaD-MoaE complex from E. coli) PubMed

• UniProt: O31705

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: mobA-moeB-moeA-mobB-moaE-moaD PubMed

• Expression browser: moaE PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Tadhg P Begley, Steven E Ealick, Fred W McLafferty
Thiamin biosynthesis: still yielding fascinating biological chemistry.
Biochem Soc Trans: 2012, 40(3);555-60
[PubMed:22616866] [WorldCat.org] [DOI] (I p)

Original publications

Michael J Rudolph, Margot M Wuebbens, Oliver Turque, K V Rajagopalan, Hermann Schindelin
Structural studies of molybdopterin synthase provide insights into its catalytic mechanism.
J Biol Chem: 2003, 278(16);14514-22
[PubMed:12571227] [WorldCat.org] [DOI] (P p)