NadE

• Description: NH3-dependent NAD+ synthetase
  
  

Gene name	nadE
Synonyms	outB, tscBGH, gsp-81
Essential	yes PubMed
Product	NH3-dependent NAD+ synthetase
Function	NAD biosynthesis
MW, pI	30 kDa, 4.888
Gene length, protein length	816 bp, 272 aa
Immediate neighbours	ycgI, tmrB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, general stress proteins (controlled by SigB), essential genes

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU03130

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + deamido-NAD⁺ + NH₃ = AMP + diphosphate + NAD⁺ (according to Swiss-Prot)

• Protein family: NAD synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1KQP

• UniProt: P08164

• KEGG entry: [3]

• E.C. number: 6.3.1.5

Expression and regulation

• Operon: nadE PubMed

• Expression browser: nadE PubMed

• Sigma factor: SigA, SigB PubMed

• Regulation: induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

James A Mobley, Anton Poliakov
Detection of early unfolding events in a dimeric protein by amide proton exchange and native electrospray mass spectrometry.
Protein Sci: 2009, 18(8);1620-7
[PubMed:19554628] [WorldCat.org] [DOI] (I p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

H Antelmann, R Schmid, M Hecker
The NAD synthetase NadE (OutB) of Bacillus subtilis is a sigma B-dependent general stress protein.
FEMS Microbiol Lett: 1997, 153(2);405-9
[PubMed:9271869] [WorldCat.org] [DOI] (P p)

T Caramori, S Calogero, A M Albertini, A Galizzi
Functional analysis of the outB gene of Bacillus subtilis.
J Gen Microbiol: 1993, 139(1);31-7
[PubMed:8450306] [WorldCat.org] [DOI] (P p)