Sandbox

• Description: replication initiation protein
  
  

Gene name	dnaA
Synonyms	dnaH, dnaJ, dnaK
Essential	yes PubMed
Product	replication initiation protein
Function	DNA replication
Interactions involving this protein in SubtInteract: DnaA
MW, pI	50 kDa, 6.035
Gene length, protein length	1338 bp, 446 aa
Immediate neighbours	rpmH, dnaN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU00010

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: dnaA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: AAA+ domain

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • SirA displaces DnaA from the replication origin PubMed
  • YabA inhibits co-operative binding of DnaA to the oriC DNA PubMed
  • DnaA helix formation (and thus replication initiation) is inhibited by the interaction of Soj with the AAA+ domain of DnaA PubMed

• Interactions:
  • DnaA assembles into a right-handed helical oligomer built upon interactions between neighbouring AAA+ domains
  • Soj-DnaA PubMed
  • DnaA-YabA, DnaA-YabA-DnaN PubMed
  • DnaA-DnaD PubMed
  • SirA-DnaA PubMed
  • YqaH-DnaA PubMed

• Localization: throughout the cytoplasm PubMed

Database entries

• Structure:

• UniProt: P05648

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dnaA-dnaN PubMed

• Expression browser: dnaA PubMed

• Sigma factor: SigA PubMed

• Regulation: negatively controlled by DnaA PubMed and Spo0A PubMed
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Philippe Noirot, Jouy-en-Josas, France homepage

Peter Graumann, Freiburg University, Germany homepage

Alan Grossman, MIT, Cambridge, MA, USA

References

Reviews

Alan C Leonard, Julia E Grimwade
Regulation of DnaA assembly and activity: taking directions from the genome.
Annu Rev Microbiol: 2011, 65;19-35
[PubMed:21639790] [WorldCat.org] [DOI] (I p)

Alan C Leonard, Julia E Grimwade
Regulating DnaA complex assembly: it is time to fill the gaps.
Curr Opin Microbiol: 2010, 13(6);766-72
[PubMed:21035377] [WorldCat.org] [DOI] (I p)

Tsutomu Katayama, Shogo Ozaki, Kenji Keyamura, Kazuyuki Fujimitsu
Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC.
Nat Rev Microbiol: 2010, 8(3);163-70
[PubMed:20157337] [WorldCat.org] [DOI] (I p)

The DnaA regulon

Alexi I Goranov, Luba Katz, Adam M Breier, Christopher B Burge, Alan D Grossman
A transcriptional response to replication status mediated by the conserved bacterial replication protein DnaA.
Proc Natl Acad Sci U S A: 2005, 102(36);12932-7
[PubMed:16120674] [WorldCat.org] [DOI] (P p)

Original publications

Additional publications: PubMed