MenC

• Description: O-succinylbenzoate synthase
  
  

Gene name	menC
Synonyms	ytfD
Essential	yes PubMed
Product	O-succinylbenzoate synthase
Function	biosynthesis of menaquinone
Metabolic function and regulation of this protein in SubtiPathways: Menaquinone
MW, pI	41 kDa, 5.888
Gene length, protein length	1113 bp, 371 aa
Immediate neighbours	mntA, menE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis of cofactors, essential genes

The gene

Basic information

• Locus tag: BSU30780

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H₂O (according to Swiss-Prot)

• Protein family: mandelate racemase/muconate lactonizing enzyme family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34514

• KEGG entry: [3]

• E.C. number: 4.2.1.113

Expression and regulation

• Operon:
  • menF-menD-ytxM-menB-menE-menC PubMed
  • menB-menE-menC PubMed
  • menE-menC PubMed

• Expression browser: menC PubMed

• Sigma factor:
  • menF: SigA PubMed
  • menB: SigA PubMed
  • menE: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • A ncRNA is predicted between mntA and menC PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

D R Palmer, J B Garrett, V Sharma, R Meganathan, P C Babbitt, J A Gerlt
Unexpected divergence of enzyme function and sequence: "N-acylamino acid racemase" is o-succinylbenzoate synthase.
Biochemistry: 1999, 38(14);4252-8
[PubMed:10194342] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)

X Qin, H W Taber
Transcriptional regulation of the Bacillus subtilis menp1 promoter.
J Bacteriol: 1996, 178(3);705-13
[PubMed:8550504] [WorldCat.org] [DOI] (P p)

J R Driscoll, H W Taber
Sequence organization and regulation of the Bacillus subtilis menBE operon.
J Bacteriol: 1992, 174(15);5063-71
[PubMed:1629163] [WorldCat.org] [DOI] (P p)