MenE

• Description: o-succinylbenzoate-CoA ligase
  
  

Gene name	menE
Synonyms
Essential	yes PubMed
Product	o-succinylbenzoate-CoA ligase
Function	biosynthesis of menaquinone
Metabolic function and regulation of this protein in SubtiPathways: Menaquinone
MW, pI	54 kDa, 5.466
Gene length, protein length	1458 bp, 486 aa
Immediate neighbours	menC, menB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis of cofactors, essential genes

The gene

Basic information

• Locus tag: BSU30790

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + 2-succinylbenzoate + CoA = AMP + diphosphate + 2-succinylbenzoyl-CoA (according to Swiss-Prot)

• Protein family: MenE type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P23971

• KEGG entry: [3]

• E.C. number: 6.2.1.26

Expression and regulation

• Operon:
  • menF-menD-ytxM-menB-menE-menC PubMed
  • menB-menE-menC PubMed
  • menE-menC PubMed

• Sigma factor:
  • menF: SigA PubMed
  • menB: SigA PubMed
  • menE: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)

X Qin, H W Taber
Transcriptional regulation of the Bacillus subtilis menp1 promoter.
J Bacteriol: 1996, 178(3);705-13
[PubMed:8550504] [WorldCat.org] [DOI] (P p)

J R Driscoll, H W Taber
Sequence organization and regulation of the Bacillus subtilis menBE operon.
J Bacteriol: 1992, 174(15);5063-71
[PubMed:1629163] [WorldCat.org] [DOI] (P p)