GmuG

• Description: beta-1,4-mannanase
  
  

Gene name	gmuG
Synonyms	ydhT
Essential	no
Product	beta-1,4-mannanase
Function	glucomannan utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	40 kDa, 5.628
Gene length, protein length	1086 bp, 362 aa
Immediate neighbours	gmuF, ydhU/1
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AbrB regulon, CcpA regulon, GmuR regulon

The gene

Basic information

• Locus tag: BSU05880

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 26 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

• Structure: 2QHA, 3CBW mutant variant

• UniProt: O05512

• KEGG entry: [3]

• E.C. number: 3.2.1.78

Expression and regulation

• Operon: gmuB-gmuA-gmuC-gmuD-gmuR-gmuE-gmuF-gmuG PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by conjac glucomannan (requires GmuG) (GmuR) PubMed
  • induced by cellobiose (GmuR) PubMed
  • subject to carbon catabolite repression (CcpA) PubMed

• Regulatory mechanism:
  • GmuR: transcription repression PubMed
  • CcpA: transcription repression PubMed
  • AbrB: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed