PnpA
- Description: polynucleotide phosphorylase, RNase, involved in double-strand break repair
| Gene name | pnpA |
| Synonyms | comR |
| Essential | no |
| Product | polynucleotide phosphorylase (PNPase) (EC 2.7.7.8) |
| Function | DNA repair, competence development, RNA degradation |
| Interactions involving this protein in SubtInteract: PnpA | |
| MW, pI | 77 kDa, 4.89 |
| Gene length, protein length | 2115 bp, 705 aa |
| Immediate neighbours | rpsO, ylxY |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
genetic competence, DNA repair/ recombination, Rnases
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16690
Phenotypes of a mutant
- The pnpA mutant is cold sensitive and sensitive to tetracyclin, it shows multiseptate filamentous growth. PubMed
- The mutant is deficient in genetic competence (no expression of the late competence genes) PubMed
- The mutant overexpresses the trp and putB-putC-putP operons.
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- 3'-5' exoribonuclease, RNase
- PNPase degrades the trp mRNA from the RNA-TRAP complex
- involved in double-strand break (DSB) repair via homologous recombination (HR) or non-homologous end-joining (NHEJ) PubMed
- degrades ssDNA (3' --> 5') (stimulated by RecA, inhibited by SsbA) PubMed
- can polymerize ssDNA at a free 3' OH end, stimulated by RecN PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 3CDI (protein from E. coli), 3GCM (protein from E. coli, PNPase/RNase E micro-domain/RNA tetragonal crystal form )
- UniProt: P50849
- KEGG entry: [2]
- E.C. number:
Additional information
required for the expression of late competence genes comGA and comK, requirement bypassed by a mecA disruption; may be necessary for modification of the srfAA transcript (stabilization or translation activation)
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP584 (aphA3), available in Stülke lab
- Expression vector:
- for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP838, available in Stülke lab
- for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP1342, available in Stülke lab
- for chromosomal expression of PnpA-Strep (cat): GP1002, available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
David Bechhofer, Mount Sinai School, New York, USA Homepage
Your additional remarks
References
Reviews
José M Andrade, Vânia Pobre, Inês J Silva, Susana Domingues, Cecília M Arraiano
The role of 3'-5' exoribonucleases in RNA degradation.
Prog Mol Biol Transl Sci: 2009, 85;187-229
[PubMed:19215773]
[WorldCat.org]
[DOI]
(P p)
Sue Lin-Chao, Ni-Ting Chiou, Gadi Schuster
The PNPase, exosome and RNA helicases as the building components of evolutionarily-conserved RNA degradation machines.
J Biomed Sci: 2007, 14(4);523-32
[PubMed:17514363]
[WorldCat.org]
[DOI]
(P p)
Devanand Sarkar, Paul B Fisher
Polynucleotide phosphorylase: an evolutionary conserved gene with an expanding repertoire of functions.
Pharmacol Ther: 2006, 112(1);243-63
[PubMed:16733069]
[WorldCat.org]
[DOI]
(P p)
A J Carpousis, N F Vanzo, L C Raynal
mRNA degradation. A tale of poly(A) and multiprotein machines.
Trends Genet: 1999, 15(1);24-8
[PubMed:10087930]
[WorldCat.org]
[DOI]
(P p)
Original publications
Additional publications: PubMed
PNPase in E. coli
