Fra

• Description: frataxin-like protein, required for the biosynthesis of iron-sulfur clusters
  
  

Gene name	fra
Synonyms	ydhG
Essential	no
Product	frataxin-like protein
Function	intracellular iron channeling, biosynthesis of iron-sulfur clusters
MW, pI	14 kDa, 5.377
Gene length, protein length	369 bp, 123 aa
Immediate neighbours	phoB, ydhH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

iron metabolism

The gene

Basic information

• Locus tag: BSU05750

Phenotypes of a mutant

• severe growth phenotype associated with a broadly disturbed iron homeostasis PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transfers iron onto the B. subtilis SUF system (SufU-SufS) for iron-sulfur cluster biosynthesis PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Fe(2+) is bound with high affinity PubMed

• Effectors of protein activity:

• Interactions:
  • the protein is active as a dimer PubMed
  • (Fra)2-SufU, requires iron PubMed

• Localization:

Database entries

• Structure: 2OC6

• UniProt: Q797E6

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: available in Mohamed Marahiel's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alexander G Albrecht, Hannes Landmann, David Nette, Olaf Burghaus, Florian Peuckert, Andreas Seubert, Marcus Miethke, Mohamed A Marahiel
The frataxin homologue Fra plays a key role in intracellular iron channeling in Bacillus subtilis.
Chembiochem: 2011, 12(13);2052-61
[PubMed:21744456] [WorldCat.org] [DOI] (I p)

Wenbin Qi, J A Cowan
A structural and functional homolog supports a general role for frataxin in cellular iron chemistry.
Chem Commun (Camb): 2010, 46(5);719-21
[PubMed:20087498] [WorldCat.org] [DOI] (I p)