Spo0F

• Description: phosphotransferase of the sporulation initiation phosphorelay
  
  

Gene name	spo0F
Synonyms
Essential	no
Product	phosphotransferase of the sporulation initiation phosphorelay
Function	initiation of sporulation
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	14 kDa, 4.697
Gene length, protein length	372 bp, 124 aa
Immediate neighbours	fbaA, ywjG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

phosphorelay, phosphoproteins

This gene is a member of the following regulons

SigH regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU37130

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on an Asp residue by KinA, KinB, KinC, KinD, or KinE
  • dephosphorylated by RapA, RapB, RapE, or RapH PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: Spo0F-Spo0B PubMed, Spo0F-Spo0E PubMed, RapA-Spo0F PubMed, RapB-Spo0F PubMed, RapE-Spo0F, RapH-Spo0F PubMed, KinA-Spo0F, KinB-Spo0F, KinC-Spo0F, KinD-Spo0F, KinE-Spo0F

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:
  • 1SRR (phosphatase resistant mutant)
  • 1F51 (complex with Spo0B)
  • 2JVK (Mutant L66A),
  • 2FSP
  • 3Q15 (RapH-Spo0F complex) PubMed

• UniProt: P06628

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spo0F PubMed

• Sigma factor: SigA PubMed, SigH PubMed

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed