Obg
- Description: write here
| Gene name | obg |
| Synonyms | |
| Essential | Yes |
| Product | GTPase activity |
| Function | may be required to stimulate activity of the phosphorelay that activates Spo0A |
| MW, pI | 47 kDa, 4.859 |
| Gene length, protein length | 1284 bp, 428 aa |
| Immediate neighbours | spo0B, pheB |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
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Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
Binds and hydrolyzes GTP and readily exchanges GDP for GTP
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
Obg possesses 3 domains: A N-terminal glycine-rich domain (1–158), a GTP binding domain (159–342)and a C-terminal TGS domain (343–428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.
- Modification:
- Cofactor(s):
GDP, GTP and probably ppGpp
- Effectors of protein activity:
- Interactions:
Interaction with Rsb proteins.
- Localization:
Database entries
- Structure:
- Swiss prot entry:
- KEGG entry:
- E.C. number:
Additional information
The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in B. subtilis.
Expression and regulation
- Operon:
spo0B-obg
- Sigma factor:
One SigA-dependent promoter upstream of spo0B
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Buglino J., Shen V., Hakiman P. & Lima C.D. (2002) Structural and biochemical analysis of the Obg GTP binding protein Structure 10: 1581-1592. [5]
- Scott, J.M., Ju, J., Mitchell, T., and Haldenwang, W.G. (2000) The Bacillus subtilis GTP binding protein obg and regulators of the sigma(B) stress response transcription factor cofractionate with ribosomes J. Bacteriol. 182: 2771–2777. [6]
